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murine fibroblast cell line nih 3t3  (ATCC)


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    ATCC murine fibroblast cell line nih 3t3
    Murine Fibroblast Cell Line Nih 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nih+3t3+murine+fibroblasts/NIH%2F3T3/pm42767387-49-1-16
    Average 99 stars, based on 13280 article reviews
    murine fibroblast cell line nih 3t3 - by Bioz Stars, 2026-10
    99/100 stars

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    Modification:

    Article Title: Mus Musculus papillomavirus MmuPV1 resists restriction by human APOBEC3B
    Article Snippet: .. NIH-3T3 murine fibroblasts (ATCC, CRL-1658) were grown in Dulbecco’s modified Eagle medium (DMEM, Invitrogen, 11960–069) supplemented with 5% fetal bovine serum (ThermoFisher Scientific, A4736401). ..

    Article Title: Mus musculus papillomavirus MmuPV1 resists restriction by human APOBEC3B
    Article Snippet: .. NIH-3T3 murine fibroblasts (ATCC, CRL-1658) were grown in Dulbecco’s modified Eagle medium (DMEM, Invitrogen, 11960-069) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific, A4736401). ..

    Cell Culture:

    Article Title: 3D Printing of Oxygen-Sensing ECM-Based Skin Graft for Personalized Treatment of Chronic Wounds—A Technological Proof of Concept
    Article Snippet: Cell culture: The immortalized human keratinocyte cell line HaCaT was cultured in DMEM (Merck) supplemented with 10% FBS (Biowest, Nuaillé, France), 2 mM L-glutamine (Gibco, ThermoFisher Scientific, Waltham, MA, USA), and 1% penicillin–streptomycin. .. NIH/3T3 murine fibroblasts (CRL-1658TM, ATCC) were cultured in DMEM containing 10% FBS and 1% penicillin–streptomycin. ..

    Article Title: 3D Printing of Oxygen-Sensing ECM-Based Skin Graft for Personalized Treatment of Chronic Wounds—A Technological Proof of Concept
    Article Snippet: Cell culture: The immortalized human keratinocyte cell line HaCaT was cultured in DMEM (Merck) supplemented with 10% FBS (Biowest, Nuaillé, France), 2 mM L-glutamine (Gibco, ThermoFisher Scientific, Waltham, MA, USA), and 1% penicillin–streptomycin. .. NIH/3T3 murine fibroblasts (CRL-1658TM, ATCC) were cultured in DMEM containing 10% FBS and 1% penicillin–streptomycin. ..

    In Vitro:

    Article Title: Characterization of chitosan matrix associated with madecassoside for skin healing
    Article Snippet: and internal environment, thereby facilitating the entry of pathogens into the body.. Tissue injuries can be either acute or chronic, with the latter persisting for more than six weeks, posing a significant challenge in terms of healing and often necessitating prolonged hospitalization and complex treatments (Isaac et al. 2010; Fráguas et al. 2015).. Wound healing, a fascinating and intricate process, involves a complex interplay between various cellular constituents of the skin and the extracellular matrix.



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    murine fibroblast cell line nih 3t3  (ATCC)
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    ATCC murine fibroblast cell line nih 3t3
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    murine nih 3t3 fibroblasts  (ATCC)
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    Assessment of cell metabolic activity using the resazurin reduction assay <t>in</t> <t>NIH/3T3</t> fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from three independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as **p < 0.0021 compared with untreated control cells.
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    Assessment of cell metabolic activity using the resazurin reduction assay <t>in</t> <t>NIH/3T3</t> fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from three independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as **p < 0.0021 compared with untreated control cells.
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    Assessment of cell metabolic activity using the resazurin reduction assay <t>in</t> <t>NIH/3T3</t> fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from three independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as **p < 0.0021 compared with untreated control cells.
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    murine fibroblast cells  (ATCC)
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    Assessment of cell metabolic activity using the resazurin reduction assay <t>in</t> <t>NIH/3T3</t> fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from three independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as **p < 0.0021 compared with untreated control cells.
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    murine fibroblasts  (ATCC)
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    Effect of the aqueous leaf extract of L. rectus (LRAE) on cell viability of ( A ) HaCaT keratinocytes and ( B ) <t>NIH/3T3</t> <t>fibroblasts</t> through resazurin assay. The results are expressed as a percentage of cell viability compared to the control. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, compared to the control.
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    normal murine fibroblast cell line nih 3t3  (ATCC)
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    ATCC normal murine fibroblast cell line nih 3t3
    Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells <t>(NIH-3T3)</t> following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).
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    Assessment of cell metabolic activity using the resazurin reduction assay in NIH/3T3 fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from three independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as **p < 0.0021 compared with untreated control cells.

    Journal: Frontiers in Pharmacology

    Article Title: In vitro pharmacological evaluation of the safety and antibacterial efficacy of a Chilean honey hydrogel for veterinary wound applications

    doi: 10.3389/fphar.2026.1923388

    Figure Lengend Snippet: Assessment of cell metabolic activity using the resazurin reduction assay in NIH/3T3 fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from three independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as **p < 0.0021 compared with untreated control cells.

    Article Snippet: Murine NIH/3T3 fibroblasts were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Activity Assay, Incubation, Standard Deviation, Analysis, Control

    Assessment of nitrite production using the Griess method in NIH/3T3 fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from four independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as ****p < 0.0001 compared with LPS-stimulated control cells.

    Journal: Frontiers in Pharmacology

    Article Title: In vitro pharmacological evaluation of the safety and antibacterial efficacy of a Chilean honey hydrogel for veterinary wound applications

    doi: 10.3389/fphar.2026.1923388

    Figure Lengend Snippet: Assessment of nitrite production using the Griess method in NIH/3T3 fibroblasts incubated under different experimental conditions. Data represent the mean ± standard deviation from four independent experiments. Statistical analysis was performed using one-way ANOVA. Statistical significance was defined as ****p < 0.0001 compared with LPS-stimulated control cells.

    Article Snippet: Murine NIH/3T3 fibroblasts were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Incubation, Standard Deviation, Analysis, Control

    Effect of the aqueous leaf extract of L. rectus (LRAE) on cell viability of ( A ) HaCaT keratinocytes and ( B ) NIH/3T3 fibroblasts through resazurin assay. The results are expressed as a percentage of cell viability compared to the control. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, compared to the control.

    Journal: Plants

    Article Title: Phytochemistry and Wound-Healing, Enzyme-Inhibitory, and Antifungal Activities of the Wild Forage Legume Lotus rectus L.

    doi: 10.3390/plants15091367

    Figure Lengend Snippet: Effect of the aqueous leaf extract of L. rectus (LRAE) on cell viability of ( A ) HaCaT keratinocytes and ( B ) NIH/3T3 fibroblasts through resazurin assay. The results are expressed as a percentage of cell viability compared to the control. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, compared to the control.

    Article Snippet: Human keratinocytes (HaCaT cell line, CLS Cell Lines Service GmbH, Cytion, Eppelheim, Germany), originally described by Boukamp et al. [ ], and murine fibroblasts (NIH/3T3, ATCC CRL-1658, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; 41965-039, Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% ( v / v ) heat-inactivated fetal bovine serum (FBS; A5256701, Gibco) and 1% ( v / v ) penicillin–streptomycin (15070-063, Gibco).

    Techniques: Resazurin Assay, Control, Standard Deviation, Comparison

    Evaluation of the wound healing effect of the aqueous leaf extract of Lotus rectus L. (LRAE) on NIH/3T3 fibroblasts (Scratch assay). Data are expressed as a comparison, as a percentage of wound closure relative to control, between the wound area at 0 and 18 hours. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, ** p < 0.01, compared to control. Magnification = 10×; scale bar = 10 µm.

    Journal: Plants

    Article Title: Phytochemistry and Wound-Healing, Enzyme-Inhibitory, and Antifungal Activities of the Wild Forage Legume Lotus rectus L.

    doi: 10.3390/plants15091367

    Figure Lengend Snippet: Evaluation of the wound healing effect of the aqueous leaf extract of Lotus rectus L. (LRAE) on NIH/3T3 fibroblasts (Scratch assay). Data are expressed as a comparison, as a percentage of wound closure relative to control, between the wound area at 0 and 18 hours. Columns and bars represent mean and standard deviation, respectively ( n = 3 independent experiments performed in duplicate). Statistical analysis was performed by one-way ANOVA followed by Dunnett’s Multiple Comparison Test: * p < 0.05, ** p < 0.01, compared to control. Magnification = 10×; scale bar = 10 µm.

    Article Snippet: Human keratinocytes (HaCaT cell line, CLS Cell Lines Service GmbH, Cytion, Eppelheim, Germany), originally described by Boukamp et al. [ ], and murine fibroblasts (NIH/3T3, ATCC CRL-1658, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM; 41965-039, Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% ( v / v ) heat-inactivated fetal bovine serum (FBS; A5256701, Gibco) and 1% ( v / v ) penicillin–streptomycin (15070-063, Gibco).

    Techniques: Wound Healing Assay, Comparison, Control, Standard Deviation

    Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

    Journal: RSC Advances

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities

    doi: 10.1039/d6ra01587e

    Figure Lengend Snippet: Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

    Article Snippet: The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).

    Techniques: Activity Assay, Inhibition, MTT Assay